BioWhittaker® X-VIVO™ 04-418Q X-VIVO™ 15 含酚紅化學(xué)成分確定的無(wú)血清造血細(xì)胞培養(yǎng)基
英文名稱：  X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium
產(chǎn)品應(yīng)用：  腫瘤浸潤(rùn)淋巴細(xì)胞，CD3+等細(xì)胞的培養(yǎng)
中國(guó)俗稱：  無(wú)血清培養(yǎng)基  中國(guó)簡(jiǎn)稱：  無(wú)血清培養(yǎng)基
注冊(cè)品牌：  BioWhittaker® X-VIVO™  生產(chǎn)廠家：  美國(guó)Lonza公司
訂購(gòu)貨號(hào)：  04-418Q-2
規(guī)格型號(hào)：  1L/瓶
保存溫度：  2～8 ℃
產(chǎn)品種類：  淋巴細(xì)胞培養(yǎng)基及相關(guān)產(chǎn)品
到貨時(shí)間：  2～5天到貨

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產(chǎn)品英文名稱：LONZA 04-418Q X-VIVO™ 15Chemically Defined, Serum-free Hematopoietic Cell Medium
產(chǎn)品中文名稱：X-VIVO™ 15化學(xué)成分確定的無(wú)血清造血干細(xì)胞培養(yǎng)基

LONZA 04-418Q X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Medium 研究背景
 Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Lonza’s philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. We have not included any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.
The products LONZA 04-418Q X-VIVO 15 are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO™ Medium products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Product Manager.

LONZA X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 簡(jiǎn)介
X-VIVO™ 20 Medium was developed and optimized to support the generation of LAK cells from monocyte-depleted PBL at high density. Initial cell densities between 2.0- 3.0 x 107 cells/ml were successfully used to generate LAK cells. X-VIVO™ Medium 20 may also be used as a growth medium for PBL and TIL.

LONZA X-VIVO™ 15化學(xué)成分確定的無(wú)血清造血細(xì)胞培養(yǎng)基特點(diǎn)
» 不含血清
» 化學(xué)成份明確
» 成份包含L-谷氨酰胺，慶大霉素和酚紅
» LONZA 04-418Q zui適于無(wú)血清環(huán)境中腫瘤浸潤(rùn)淋巴細(xì)胞(TIL)增殖

X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic Cell Medium 使用注意事項(xiàng)
1. LONZA 04-418Q X-VIVO™ 15 is similar in composition to X-VIVO™ 10 and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes (TIL) under serum-free conditions.
 2.Please see Supplemental Information for additional hematopoietic cell applications.
 3.X-VIVO™ 15, catalog number 04-418 is formulated with L-glutamine, gentamicin, and phenol red. Catalog number 04-744Q contains L-glutamine but does not contain gentamicin or phenol red.
4.X-VIVO™ 15, catalog number BE02-053Q is formulated with L-glutamine, gentamicin, recombinant transferrin, and phenol red. X-VIVO™ 15, catalog number BE02-054Q, contains L-glutamine and recombinant transferrin but does not contain gentamicin or phenol red. The recombinant transferrin replaces native transferrin.

Lonza X-VIVO™ 化學(xué)成分確定的無(wú)血清造血干細(xì)胞培養(yǎng)基訂購(gòu)信息
Catalog Number      Description                                                                                Size
LONZA 04-380Q                  X-VIVO™ 10 Medium with L-glutamine, gentamicin,                1 L
                                 and phenol red
LONZA 04-743Q                  X-VIVO™ 10 Medium with L-glutamine; without                        1 L
                                 gentamicin or phen red   
LONZA BE04-418F             X-VIVO™ 15 Medium with L-glutamine, gentamicin,                500ml
                                and phenol red (Europe only)
LONZA 04-418Q                 X-VIVO™ 15 Medium with L-glutamine, gentamicin,                 1 L
                                and phenol red
04-744Q                 X-VIVO™ 15 Medium with L-glutamine; without                         1 L
                               gentamicin orphenol red                                      
04-448Q                X-VIVO™ 20 Medium with L-glutamine, gentamicin,                  1 L
                               and phenol re

LONZA X-VIVO 15 無(wú)血清培養(yǎng)基 英文簡(jiǎn)述：
LONZA 04-418Q X-VIVO™ 15 is similar in composition to X-VIVO™ 10 and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes （TIL） under serum-free conditions.

Please see Supplemental Information for additional hematopoietic cell applications.

LONZA  X-VIVO™ 15, catalog number 04-418 is formulated with L-glutamine, gentamicin, and phenol red. Catalog number 04-744Q contains L-glutamine but does not contain gentamicin or phenol red.重慶市華雅干細(xì)胞技術(shù)有限公司在倉(cāng)庫(kù)備有現(xiàn)貨，歡迎新老客戶：     

LONZA  X-VIVO™ 15, catalog number BE02-053Q is formulated with L-glutamine, gentamicin, recombinant transferrin, and phenol red. X-VIVO™ 15, catalog number BE02-054Q, contains L-glutamine and recombinant transferrin but does not contain gentamicin or phenol red. The recombinant transferrin replaces native transferrin.

Lonza UltraCULTURE?無(wú)血清培養(yǎng)基,500ml,不含L-谷氨酰胺
英文名稱：  UltraCULTURE? Serum-free Medium
產(chǎn)品應(yīng)用：  哺乳動(dòng)物細(xì)胞培養(yǎng)，病毒顆粒生成等
中國(guó)俗稱：  無(wú)血清培養(yǎng)基  中國(guó)簡(jiǎn)稱：  無(wú)血清培養(yǎng)基
注冊(cè)品牌：  Lonza  生產(chǎn)廠家：  美國(guó)Lonza公司
訂購(gòu)貨號(hào)：  12-725F
規(guī)格型號(hào)：  500ml/瓶
保存溫度：  2～8℃
產(chǎn)品種類：  無(wú)血清培養(yǎng)基
到貨時(shí)間：  上海倉(cāng)庫(kù)有現(xiàn)貨，1～5天到貨
產(chǎn)品編碼：  YS000235
產(chǎn)品英文名稱：LONZA 12-725F UltraCULTURE? Serum-free Medium
產(chǎn)品中文名稱：UltraCULTURE?無(wú)血清培養(yǎng)基

LONZA 12-725F UltraCULTURE? Serum-free Medium產(chǎn)品介紹：
LONZA 12-725F UltraCULTURE?無(wú)血清培養(yǎng)基是一個(gè)*、通用的培養(yǎng)基，設(shè)計(jì)用于各種粘附型和非粘附型哺乳動(dòng)物細(xì)胞的培養(yǎng)。UltraCULTURE?無(wú)血清培養(yǎng)基可用于雜種細(xì)胞生成過(guò)程中促進(jìn)細(xì)胞融合，促進(jìn)單核細(xì)胞、巨噬細(xì)胞、上皮細(xì)胞和成纖纖細(xì)胞系的增殖生長(zhǎng)，并且有助于疫苗生產(chǎn)中病毒顆粒的產(chǎn)生。培養(yǎng)基中加入了重組人胰島素，牛轉(zhuǎn)鐵蛋白和牛血清蛋白純化混合物。上海華雅思創(chuàng)生物公司在上海倉(cāng)庫(kù)長(zhǎng)期儲(chǔ)備現(xiàn)貨，以優(yōu)惠的價(jià)格向廣大生命科學(xué)研究員和細(xì)胞培養(yǎng)專業(yè)技術(shù)人員提供此款通用細(xì)胞治療級(jí)的LONZA 12-725F UltraCULTURE? Serum-free Medium, general purpose, without L-glutamine SFM無(wú)血清培養(yǎng)基
UltraCULTURE?培養(yǎng)基總蛋白濃度接近3mg/ml。UltraCULTURE?培養(yǎng)基可以作為防冷凍培養(yǎng)基(Cat.2-132A)補(bǔ)充物便于凍存細(xì)胞在無(wú)血清環(huán)境中培養(yǎng)。LONZA 12-725F UltraCULTURE?無(wú)血清培養(yǎng)基不含L-glutamine(L-谷氨酸鹽)；用之前添加5ml 200mM/L L-谷氨酸鹽溶液(Cat.Nos.17-605 or 17-905)。重慶市華雅干細(xì)胞技術(shù)有限公司在倉(cāng)庫(kù)備有現(xiàn)貨，歡迎新老客戶：     

LONZA UltraCULTURE? Serum-free Medium產(chǎn)品性能及應(yīng)用：
 用于粘附和非粘附性哺乳動(dòng)物細(xì)胞培養(yǎng)
 疫苗產(chǎn)品中病毒顆粒的生成
 UltraCULTURE?培養(yǎng)基中可能會(huì)出現(xiàn)混濁，但不影響產(chǎn)品性能

UltraCULTURE? Serum-free Medium英文簡(jiǎn)述：
LONZA 12-725F UltraCULTURE? Serum-free Medium is a complete, allpurpose medium designed for the c*tion of a wide variety of adherent and non-adherent mammalian cell types. UltraCULTURE? Medium can be used to support fusion of cells during hybridoma formation, growth of monocyte, macrophage, epithelial, and fibroblastic cell lines, and generation of virus particles for vaccine production. The medium is supplemented with recombinant human insulin, bovine transferrin, and a purified mixture of bovine serum proteins. The total protein concentration of UltraCULTURE? Medium is approximay 3 mg/ml. UltraCULTURE? Medium can be supplemented with Cryoprotective Medium (Cat. No. 12-132A) to cryopreserve cells in a serum-free environment. UltraCULTURE? Medium does not contain L-glutamine; please add 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior to use.

The formulation for UltraCULTURE? Medium has been submitted to the FDA as a Master File. Permission to crossreference the Master File may be obtained by contacting the Regulatory Affairs Department.

Applications
– C*tion of adherent and non-adherent mammalian cells
– Generation of viral particles for vaccine production
Performance and Quality Tests
– Turbidity may develop in UltraCULTURE? Medium; experiments have determined that the turbidity will not alter the performance of the product

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