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Anti-E Cadherin antibody (ab15148)抗E鈣黏蛋白抗體產(chǎn)品信息：
- Product nameAnti-E Cadherin antibody
- Description
Rabbit polyclonal to E Cadherin
- SpecificityWe have data to indicate that this antibody may not cross react with Chicken. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Tested applicationsWB, ICC/IF, ELISA, ICC, IHC-P, IHC-Fr
- Species reactivity
Reacts with: Human, Pig
- Immunogen
Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin.
- Positive control
- Skin
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: Sodium Azide
Constituents: 10mM PBS, BSA. pH 7.4 Concentration 500 µl at 0.2 mg/ml
- PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
- Signal Transduction
- Cytoskeleton / ECM
- Cell Adhesion
- Cadherins
- Signal Transduction
- Signaling Pathway
- Calcium Signaling
- Calcium Binding Proteins
- Cancer
- Invasion/microenvironment
- ECM
- Cell adhesion
- Cadherins
- Developmental Biology
- Organogenesis
- Excretory system development
- Kidney development
Applications
Our Abpromise guarantee covers the use of ab15148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500. Detects a band of approximay 120 kDa. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. |
| ELISA | ELISA: Use at an assay dependent concentration. |
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/30. for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
| IHC-Fr | IHC-Fr: 1/50. |
Target
- FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. - Tissue specificityNon-neural epithelial tissues.
- Involvement in diseaseDefects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Defects in CDH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in CDH1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. - Sequence similaritiesContains 5 cadherin domains.
- Post-translational
modificationsDuring apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. - Cellular localizationCell junction. Cell membrane. Endosome. Golgi apparatus > trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.
Database links
- Entrez Gene: 999 Human
- Entrez Gene: 100048953 Pig
- Omim: 192090 Human
- SwissProt: P12830 Human
- Unigene: 461086 Human
Alternative names
- Arc 1 antibody
- CADH1_HUMAN antibody
- Cadherin 1 antibody
Anti-E Cadherin antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)
Immunohistochemical staining of formalin fixed paraffin embedded human skin using ab15148.
Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)This image is courtesy of an Abreview submitted by Dr Mauricio Pinto
ab15148 staining human MCF10A cells by ICC/IF. Cells were fixed with paraformaldehyde and blocked using 10% serum for 30 minutes at 25 °C. The primary antibody was diluted 1/25 in TBST and incubated for 1 hour at 25 °C. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)This image is a courtesy of Anonymous Abreview
ab15148 staining E Cadherin in human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in Citrate pH 6.0 and then blocking with 5% serum for 1 hour at 23°C was performed. The primary antibody was used diluted 1/50 and incubated with sample for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview
ab15148 staining E Cadherin in Pig Cervix uteri tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% goat serum for 1 hour at 37°C; antigen retrieval was by heat mediation in 10mM citrate at pH 6 for 2 minutes. The sample was incubated with primary antibody (1/50) at 4°C for 12 hours. An HRP-conjugated goat polyclonal to rabbit IgG (undiluted) was used as secondary antibody.
Immunocytochemistry/ Immunofluorescence - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview
ab15148 staining E cadherin in Human AGS Gastric carcinoma cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilzed with 0.025% Trton X-100 in TBS and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - E Cadherin antibody (ab15148)This image is courtesy of an anonymous Abreview.
ab15148 staining E Cadherin in Human AGS Gastric Carcinoma tissue sections by Immunohistochemistry (Frozen sections). The sections were acetone fixed and blocked in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/50 in blocking buffer and incubated with the sample for 1 hours at 23°C. An HRP-conjugated Goat polyclonal to Rabbit IgG, diluted 1/200, was used as the secondary.
- Western blot - E Cadherin antibody (ab15148)
All lanes : Anti-E Cadherin antibody (ab15148) at 1/500 dilution (for 16 hours at 4°C)
Lane 1 : Human OE33 cell - whole cell lysate
Lane 2 : Human OE33 cell - whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 120 kDa 公司介紹：
Abcam位于英國的劍橋科學園，成立于1998年，專門生產(chǎn)和分銷研究型抗體。主要創(chuàng)立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(chinorino.com) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。
Abcam 一直致力加強產(chǎn)品線，為使研究員更容易找到蛋白質研究試劑產(chǎn)品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產(chǎn)品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。
Abcam 的 目標是給世界上的抗體建立zui大的在線目錄，為各地科學家提供*產(chǎn)品，成為各國科學界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發(fā)布有關我們 每一種產(chǎn)品的盡可能多的信息。
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