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| 品牌 | 其他品牌 | 貨號 | Y00185/Y00275/Y00285/Y00315/Y00325/Y0030 |
|---|---|---|---|
| 規(guī)格 | 套裝 | 供貨周期 | 一個月 |
| 主要用途 | 細胞治療、藥物監(jiān)測和疾病模型等 |
重慶市華雅干細胞技術有限公司 ， ：
華雅集團旗下授權代理TTAKARA Cellartis干細胞產(chǎn)品
圖片為展示效果。實際產(chǎn)品為細胞株+培養(yǎng)基套裝
人iPS誘導多能干細胞
(Human Induced Pluripotent Stem Cells)
產(chǎn)品英文名稱：Cellartis® Human iPS Cell Lines
產(chǎn)品中文名稱：Cellartis® 人iPS誘導多能干細胞
Cellartis人iPS細胞（Human iPS Cell Line）產(chǎn)品是達到了工業(yè)客戶使用標準生產(chǎn)的干細胞產(chǎn)品。該產(chǎn)品包括細胞和用于增殖培養(yǎng)的Cellartis DEF-CS Culture System培養(yǎng)體系。iPS細胞已經(jīng)在制品化時完成了對DEF-CS培養(yǎng)體系的適應。Cellartis DEF-CS Culture System培養(yǎng)體系是組分明確而且不依賴飼養(yǎng)層的便捷的干細胞培養(yǎng)體系。Cellartis DEF-CS Culture System適合高效率地增殖培養(yǎng)干細胞，并且保持干細胞在增殖過程中幾乎無分化地生長，這使得不需要在大規(guī)模增殖培養(yǎng)過程中進行細胞篩選。因此，Cellartis人iPS細胞（Human iPS Cell Line）產(chǎn)品是一套完整的產(chǎn)品體系，對于科研級別和工業(yè)規(guī)模的需求都適合。
Cellartis人iPS誘導多能干細胞特點
☆細胞系通過使用缺陷型多順反子逆轉錄病毒（defective polycistronic retrovirus）技術重編程人皮膚成纖維細胞以轉入Oct-4，SOX2，KLF4和c-Myc
☆細胞系的純度和干細胞特征經(jīng)過廣泛分析，包括解凍后的恢復，支原體和細菌檢測，干細胞特異性標記物的表達（Oct-4，NANOG，SSEA-3，SSEA-4，TRA-1 -60，TRA-1-81），不表達分化標記（β-3微管蛋白，F(xiàn)OXA2，ASMA）和預期核型
☆用Cellartis DEF-CS培養(yǎng)系統(tǒng)優(yōu)化培養(yǎng)，試劑盒包括Cellartis DEF-CS 100培養(yǎng)系統(tǒng)
☆適用于所有主要應用; 細胞在分化時可以分化形成所有三個胚層的衍生物
Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc
Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype
Optimized for culture with the Cellartis DEF-CS Culture System
Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated
Kits include the Cellartis DEF-CS 100 Culture System
Cellartis 人iPS誘導多能干細胞參數(shù)
不同貨號對應供體年齡性別冷凍代數(shù)和已確認分化能力
iPS誘導多能干細胞的應用
細胞治療、藥物監(jiān)測和疾病模型
Cellartis 人iPS誘導多能干細胞組份
不同供體的iPS細胞系和DEF-CS 100*培養(yǎng)基
*培養(yǎng)基包含基礎培養(yǎng)基、添加劑和包被劑。
保存：細胞 ≤ –150°C，添加劑 –20°C,基礎培養(yǎng)基和包被劑4 °C。
A. Cellartis Human iPS Cell Lines
Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185)
Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225)
Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265)
Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275)
Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285)
Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305)
Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315)
Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325)
B. Cellartis DEF-CS 100 Culture System
· Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.)
o DEF-CS Basal Medium (100 ml)
o DEF-CS COAT-1 (for 100 ml) (800 µl)
o DEF-CS GF-1 (for 100 ml) (300 µl)
o DEF-CS GF-2 (for 100 ml) (100 µl)
o DEF-CS GF-3 (for 100 ml) (40 µl)
另行準備材料：PBS+TrypLE 選擇酶+TC處理ps表面培養(yǎng)器皿
The following materials are required but not supplied:
· PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)
· PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–)
· TrypLE Select Enzyme (1X), no phenol red
· Cell culture vessels, Tissue culture treated polystyrene surface
· General cell culture equipment used in cell culture laboratory
參考文獻：
1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.
2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.
3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.
4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.
Cellartis 人iPS誘導多能干細胞訂購信息
不同貨號的區(qū)別：iPS細胞供體不同
品牌 | Code No. | 包裝量 | 價格 |
Cellartis | Y00185 | 1 Kit | 22718 元 |
Cellartis | Y00275 | 1 Kit | 22718 元 |
Cellartis | Y00285 | 1 kit | 22718 元 |
Cellartis | Y00315 | 1 Kit | 22718 元 |
Cellartis | Y00325 | 1 kit | 22718 元 |
Cellartis | Y00305 | 1 Kit | 22718 元 |
?華雅集團旗下授權代理TTAKARA Cellartis干細胞產(chǎn)品，Cellartis的多能細胞培養(yǎng)系統(tǒng)包括iPS誘導多能干細胞株、iPS誘導多能干細胞和ES胚胎干細胞培養(yǎng)基，iPS基因編輯用單細胞克隆培養(yǎng)基和多能細胞檢測用單克隆抗體。
重慶市華雅干細胞技術有限公司以“專注干細胞研究，提升干細胞品質(zhì)”為發(fā)展理念，專業(yè)從事干細胞基礎研究、干細胞再生醫(yī)學臨床應用研究及干細胞技術服務，自主研發(fā)產(chǎn)品熱賣中：人間充質(zhì)干細胞無血清培養(yǎng)基
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